紫外交聯儀 Stratalinker UV 2400
特點:
為了使核苷酸固定在膜上,傳統的方法是將膜置于真空烘箱中80度烘2小時,在紫外交聯儀上僅需30-60秒
與真空烘焙相比,增加雜交信號強度
內部配備光度檢測儀,可以補償由于紫外燈泡老化造成的輸出偏移
具自動交聯、時間和能量三種模式供選擇
帶有顯示時間、能量的顯示器及設置面板
紫外交聯儀 Stratalinker UV 2400
應用:
將核酸共價結合到固相支持物(如硝酸纖維素膜)上
Northem,Southern,dot及slot印跡分析
雜交芯片的制備
菌落、噬菌斑篩選,瓊脂糖凝膠中DNA的切割
產生胸腺二聚體,用于部分限制性內切酶消化作圖
紫外敏感性測試,用于鑒定recA宿主菌
樣品紫外照射處理
誘導培養細胞的凋亡
照射波長:254nm(配8瓦254nm燈泡5個)
內部尺寸:47.7W/34.3D/16.8H
紫外交聯儀 Stratalinker UV 2400
Stratagene UV Stratalinker 2400
The Stratagene UV Stratalinker 2400 crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crosslinking performance, each Stratalinker UV crosslinker is equipped with aninternal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs. The Stratalinker UV crosslinker may be used for Northern, Southern, dot or slot blot analysis, colony or plaque screening, nicking of DNA in agarose gels prior to blotting, dimer formation to perform partial digests for rapid restriction mapping, UV sensitivity testing for host strain verification, and UV irradiation of PCR samples. The Stratalinker UV crosslinker may be used in the Autocrosslink,Time, or Energy modes.